PCR product sequencing savior

So this link has really helped me when I stuck with interpreting what the "N" were in my PCR product sequencing result. Thank you very much <3

https://seqcore.brcf.med.umich.edu/sites/default/files/html/interpret.html

I literally planned to run another PCR with high fidelity polymerase

My PCR product was some mutation in the human genomic DNA, my template was the same and the mutation was hetergeneous.

I just went back to my chromatograms and yo! done confirmed my base :)

TPP_5.1.0-src - make install - error

https://groups.google.com/forum/#!topic/spctools-discuss/G51KeZSq9P8

https://groups.google.com/forum/#!topic/spctools-discuss/o3sqfB1_QEs

> Error in finding FindBin::libs module perl
install through cpan

cpan
install FindBin::libs

 > Check PERL modules for installation and in case problem with .pm file location move them to the default detection of lib/perl

https://www.mail-archive.com/search?l=spctools-discuss@googlegroups.com&q=subject:%22Re%5C%3A+%5C%5Bspctools%5C-discuss%5C%5D+tpp_gui.pl%5C%3A+Error%5C%3A+invoking+tpp_hostname+to+look+up+installation+property+%27HomePath%5C%21%27%22&o=newest&f=1

> Run TPP as sudo
Run  any command from /usr/local/tpp/bin

Problem running 32-bit tools on WINE _ LINUX Ubuntu

Courtesy/Reference : https://linux-tips.com/t/couldnt-run-64-bit-programs-with-wine/320

This helps

$ WINEARCH=win64 WINEPREFIX=~/.wine64 winecfg

$ WINEPREFIX=~/.wine64 wine app.exe